Determination of transcription starting site of Mus musculus Melanocortin 3 Receptor gene using “New 5’RACE”

TSS (Transcription starting site) determination is key to the study of transcriptional regulation, and “New 5´ RACE” (rapid amplification of cDNA ends) is a challenge method for determining multiple transcription starting sites . Since Melanocortin 3 receptor (MC3R) plays an important role in the regulation of body obesity, protocol for obtaining 5´UTR Transcription starting site of mouse Melanocortin 3 Receptor is presented.