REMEDi4ALL - Standard Operating Procedure - Cellular Thermal Shift Assay (CETSA) for target engagement, screening or medicinal chemistry

Evaluating the binding of a compound to its putative target is an important step in drug discovery and development. Without this critical information the relationship between the small molecule, the protein target and modulation of disease biology cannot be adequately studied in vitro or in vivo. The cellular thermal shift assay (CETSA) is one important method to monitor target engagement, which has many potential applications to the drug discovery and development pipeline. This SOP outlines the use of CETSA for measuring target engagement for target validation, screening or structure-activity relationship studies.