Published June 27, 2025 | Version v1.0.1

Inferring Exon and Intron Metadata from .gff file

  • 1. ROR icon University of Toronto

Description

This pipeline infers exon functional annotations using coordinates from a .gff file and derives corresponding intron coordinates along with relevant annotations. Users can optionally specify the length of intronic fragments to extract. The pipeline is suited for splicing code analyses.

Notes

If you use this pipeline, please cite it - 

Bhatnagar, S., & Calarco, J. (2025). Inferring Exon and Intron Metadata from .gff file (v1.0.1). Zenodo. https://doi.org/10.5281/zenodo.15757714

Alternatively, the study that first used this can be cited - Bhatnagar, S., Ho, J., Singh, I., Sadek, N. H., et al. (2025). An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal. bioRxiv. https://doi.org/10.1101/2025.08.16.670696

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