Published June 6, 2025
| Version v2
Journal article
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A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescence
Authors/Creators
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1.
Montreal Neurological Institute and Hospital
Description
This report provides a guide to selecting high-quality commercial antibodies against the lysosomal enzyme glucocerebrosidase (GCase) for use in Western blot and immunoprecipitation, utilizing a standardized experimental protocol that compares read-outs in knockout cell lines and isogenic parental controls. This research was partly funded by the G-Can (GBA1-Canada) initiative, an open-science collaboration dedicated to advancing research on Parkinson's disease associated with GBA1 mutations. This work was also supported by the Quebec Consortium for Drug Discovery (CQDM), a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec.
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GBA1_YCharOS_Zenodo_v5.pdf
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(2.5 MB)
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