Single Nucleus RNA sequencing of the Striatum of Two Murine Parkinson's Disease Models.

This Zenodo deposit contains a publicly available description of the Dataset:

Title: "Single Nucleus RNA sequencing of the Striatum of Two Murine Parkinson's Disease Models.".

Description: This dataset includes single-nucleus RNA sequencing (snRNA-seq) data derived from mouse models of Parkinson's disease (PD), designed to capture both cell-type-specific and spatial gene expression changes across different stages and mechanisms of PD-like pathology. Single-nucleus RNA sequencing (snRNA-seq) was performed on mouse striatal tissue from two distinct PD models:

1. Mild Neurotoxin Model (6-OHDA): Adult wild-type mice received unilateral injections of 6-hydroxydopamine (6-OHDA) at a low dose (0.6 µg/µL) into the medial forebrain bundle (MFB), selectively damaging nigrostriatal dopaminergic neurons and inducing mild Parkinsonian pathology. Untreated wild-type mice served as controls.

2. Progressive Genetic Model (MitoPark): The MitoPark model (Slc6a3-cre; Tfam^flx/flx) induces mitochondrial dysfunction specifically in dopaminergic neurons, leading to progressive neurodegeneration. Samples were collected at two timepoints:

   • 10–11 weeks of age: Early stage, pre-symptomatic or mildly affected, and
   • 15–18 weeks of age: Late stage, with clear motor deficits and advanced degeneration. This snRNA-seq dataset enables in-depth analysis of transcriptomic alterations across distinct PD etiologies and stages.

This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual.

This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).

This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset