BARBERSHOP: A Modular, Reversible, and Ethical CRISPR-Based Framework for the Personalized Treatment of Human Alopecia

The BARBERSHOP project introduces a modular and reversible framework for the treatment of alopecia, leveraging programmable gene regulation tools such as CRISPR interference (CRISPRi) and activation (CRISPRa). Unlike irreversible genome-editing strategies, this system operates at the transcriptional level, allowing gene expression to be finely tuned and halted as needed. The approach integrates non-integrative delivery systems, tissuespecific promoters, and kill-switch mechanisms to ensure full reversibility.
This therapeutic platform addresses a wide spectrum of alopecia subtypes—including androgenetic, autoimmune, scarring, stress-induced, and neuropsychiatric forms—by stratifying patients based on molecular, immunological, and psychological parameters. Each intervention is designed to respect the patient’s biological identity and avoid aesthetic normalization.
The proposal calls for empirical validation in preclinical models and adheres to strict bioethical guidelines grounded in autonomy, consent, and justice.