QUANTITATIVE ANALYSIS OF EMBELIN IN AYURVEDIC FORMULATIONS VIDAGNASAVA BY UV SPECTROPHOTOMETRY

In this present study a new, simple, rapid, sensitive, precise and economic spectrophotometric method in ultraviolet
region has been developed for the determination of embelin (marker compound) in different marketed and In house
formulation of Vidagnasava. Embelin showed the maximum wavelength at 294.3 nm and hence the UV
spectrophotometric method was performed at 294.3 nm. The samples were prepared by solvent fraction method.
Pure embelin obeys Beers law in concentration ranges of 20-120mcg/ml. The content of embelin in ayurvedic
formulation was determined by calibration curve y = 0.007x - 0.116 R² = 0.998. The result of analysis has been
validated statistically and recovery studies confirmed the accuracy of the proposed method. Hence the proposed
method can be used for the reliable quantification of embelin in its ayurvedic formulation.
Key Words: Embelin, Vidangasava, Vidang, Embelia ribes