Published May 14, 2025 | Version v1

Additional SMLM Data from "Reinforced optical cage systems enable drift-free single-molecule localization microscopy"

  • 1. ROR icon Science and Technology Facilities Council
  • 2. ROR icon University of Oxford
  • 1. ROR icon Science and Technology Facilities Council
  • 2. ROR icon Jiangsu University of Science and Technology
  • 3. ROR icon Shanghai Institute of Optics and Fine Mechanics
  • 4. RayCage (Zhenjiang) Photoelectric Technology Co., Ltd

Description

This dataset contains additional data for the paper titled 'Reinforced optical cage systems enable drift-free single-molecule localization microscopy' that has been generated to address reviewer comments. The dataset has the following:

  1. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data. Used for axial drift and lateral drift quantification.
  2. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .tif files for images, .csv files for localization data
  3. Two STORM image series' of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a homebuilt microscope and localization data. .tif files for images, .csv files for localization data
  4. Two STORM image series' of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a commercial microscope and localization data. .tif files for images, .csv files for localization data.
  5. Two STORM image series' of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope and localization data. .tif files for images, .csv files for localization data. 
  6. Two STORM image series' of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope and localization data. .tif files for images, .csv files for localization data.

Files

d2_npc_elyra_reconstruction.csv

Files (47.7 GB)

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Additional details

Funding

Rosetrees Trust
Science and Technology Facilities Council
POCF2021-14
Science and Technology Facilities Council
ST/S005404/1
Biotechnology and Biological Sciences Research Council
BB/T008784/1

Dates

Available
2025-10-16