Relevant code for "Metformin inhibits mitochondrial complex I in intestinal epithelium to promote glycemic control"

The jejunum_RNAseq code performs differential gene expression analysis and creates an MA plot of the RNA-seq data. Aim was to determine whether expression of S. cerevisiae NDI1 in intestinal epithelium of mice altered gene expression in the jejunum.

The Diff_abundance_and_volcano code performs differential metabolite abundance analysis and creates a volcano plot of the data. Aim was to identify metformin-induced changes to the plasma metabolome in humans. Data used in the analysis are publicly available (PMID: 27378919).