The Align Foundation
Published May 8, 2025 | Version v1
Publication Open

GROQ-seq Technical Status Report – January 2025

Creators

  • 1. The Align Foundation
  • 2. ROR icon University of California, San Francisco
  • 3. ROR icon National Institute of Standards and Technology
  • 4. The City College of New York Department of Physics
  • 5. Bioptima Consultants Limited
  • 6. ROR icon The University of Texas Southwestern Medical Center
  • 7. ROR icon Rice University

Description

This technical report presents the first-year update of the GROQ-seq platform, a high-throughput, growth-based assay system designed to generate quantitative protein function data at scale. Developed in collaboration between The Align Foundation and NIST, GROQ-seq enables sequence-to-function mapping for diverse protein types using modular genetic circuits in Escherichia coli. Key engineering updates include the adoption of DHFR-based selection circuits, modular Golden Gate plasmid design, and standardized media production to improve reproducibility across multi-site experiments. The report also outlines the onboarding of seven protein function assays, supporting GROQ-seq’s role in building open-access genotype–phenotype datasets for protein engineering and predictive biology.
DOI 10.6084/m9.figshare.28963640

Files

GROQ-seq Technical Status.pdf

Files (5.3 MB)

Name Size Download all
md5:233197a89f39ceeb872b31dedb010830
5.3 MB Preview Download

Additional details

Identifiers

DOI
10.6084/m9.figshare.28963640

Related works

Is supplement to
Publication: 10.5281/zenodo.13909104 (DOI)