Published May 1, 2025 | Version 1.0
Dataset Open

FltJ DOMEK data (mRNA display-derived NGS datasets)

  • 1. The University of Tokyo
  • 2. ROR icon National University of Singapore

Contributors

Project leader:

  • 1. ROR icon The University of Tokyo

Description

NGS data (Illumina NovaSeq 6000; 150PE; S4 flow cell; single lane) for the FltJA DOMEK experiment (library r.rec.8m8). Uploaded are the .fastq.gz datasets after merging paired-end read (R1/R2) files. Each file corresponds to DNA recovered from a single mRNA display pulldown. Plese refer to the accompanying manuscript for futher details. The file names map to individual experiments as follows:

FT/E postfix: reacted/unreacted fraction dataset (FT: reacted, E: unreacted)

cxxx: enzyme concentration. c000: 0 uM enzyme, c008: 0.08 uM, c080: 0.8 uM, and c800: 8 uM FltJA. 

txxx: reaction time. t0: 0 min reaction; t15: 15 min reaction, t90: 90 min reaction, t540: 540 min reaction.

The dataset consists of 32 files in total. r_rec_8m8_v2_m.gz is an additional NGS sample for the naive r.rec.8m8 library (library pre-pulldown).

Files

Files (30.5 GB)

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md5:1c188d287d2d333978da2abeacc3b003
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Additional details

Dates

Created
2025-05-01
Initial dataset deposition

Software

Repository URL
https://github.com/avngrdv/DOMEK
Programming language
Python
Development Status
Active