REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR ANTIBACTERIAL DRUG

The rising incidence of bacterial infections and the growing challenge of antimicrobial resistance have underscored the need for accurate, reliable, and rapid analytical techniques to ensure the quality and therapeutic efficacy of antibacterial agents. High-performance liquid chromatography (HPLC), particularly reverse-phase HPLC (RP-HPLC), remains a crucial analytical tool for pharmaceutical analysis due to its precision, sensitivity, and reproducibility. This study aims to develop and validate a simple, specific, and robust RP-HPLC method for the quantification of a selected antibacterial agent in both its pure form and formulated products.

Method development involved systematic optimization of various chromatographic parameters, including the selection of the stationary phase, mobile phase composition, flow rate, and detection wavelength. A C18 reversed-phase column was employed, and an isocratic elution using a mixture of acetonitrile and water (with appropriate pH adjustment) provided well-resolved, sharp peaks with acceptable retention times. Optimal flow rate and detection wavelength were selected to enhance sensitivity and minimize peak tailing, ensuring accurate and reliable quantification.