Small wedge MicroED data of Lysozyme, low-dose MTH1-8DG and high-dose MTH1-8DG
Description
Small wedge MicroED data of Lysozyme, low-dose MTH1-8DG and high-dose MTH1-8DG.
A. Data information
Dataset 1: Lysozyme
Dataset 2: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using high fluence.
Dataset 3: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using low fluence.
B. Data collection
Data was collected with a CMOS CetaD detector installed on a Titan Krios G2 with an autoloader.
Data collection software: EPUD
Cryo-EM infrastructure unit, Scilifelab, Stockholm.
C. Instrument and measurement settings
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Instrumental: |
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Instrument |
Titan Krios G3i |
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Radiation source |
FEG |
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Accelerating voltage |
300 kV |
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Wavelength |
0.01968 Å |
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Probe Type |
Nanoprobe |
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Beam Diameter |
1.5 μm |
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Beam Convergence |
Parallel beam |
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Detector |
Ceta-D CMOS detector |
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Number of pixels in the image |
1024 x 1024 |
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Pixel size |
5.6 µm x 5.6 µm |
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Effective Camera Length |
3019.12 (lowdose), 3019.12 (highdose) and 1166 (lysozyme) |
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Hardware Binning |
4 |
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Experimental: |
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Data Type |
Electron diffraction data - 3D ED, Microcrystal diffraction (MicroED) |
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Data collection method |
Continuous rotation |
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Temperature (K) used during data collection |
77 K |
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Number of crystals contributing to the data set |
25 (lowdose), 20 (highdose), 61 (lysozyme) |
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Number of experimental frames |
20 frame/data (lowdose), 20 frame/data (highdose), 30 frame/data (lysozyme) |
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tilt range, exposure time, tilt per frame |
lowdose: 20 degree, 1s, 1 degree/frame highdose: 20 degree, 1s, 1 degree/frame lysozyme: 15 degree, 1s, 0.5 degree/frame |
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Software: |
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Software used for the data collection |
EPUD |
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Software used for processing |
AutoLEI with XDS |
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Authorship and Bibliography |
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Author(s) of the data |
Lei Wang (ESR 10) |
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Related data |
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Publication(s) |
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Files and data formats |
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Image format |
MRC |