Published August 2, 2024 | Version v1
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TABLE 1 in Tardigrades in the alpine region of Northeast China with an integrative description of Crenubiotus liangshuiensis sp. nov.

  • 1. College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, China.
  • 2. Heilongjiang Provincial Natural Resources Rights and Interests Investigation and Monitoring Institute, Harbin, 150080, Heilongjiang, China.
  • 3. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150040, Heilongjiang, China.
  • 4. College of Life Sciences, Northeast Forestry University, Harbin, 150040, Heilongjiang, China.

Description

TABLE 1. Primers and references for PCR protocols for amplification of the four DNA fragments sequenced in the study

DNA markerPrimer name and sequence (5’-3’)Primer sourcePCR programme
18S rRNASSU_F_01Medlin et al. (1988)Guidetti et al. (2013)
AACCTGGTTGATCCTGCCAGT
SSU_R_82
TGATCCTTCTGCAGGTTCACC
SSU_F_04Kiehl et al. (2007)Kiehl et al. (2007)
GCTTGTCTCAAAGATTAAGCC
SSU26_R
CATTCTTGGCAAATGCTTTCG
28S rRNA28SF0001Mironov et al.Mironov et al. (2012)
ACCCVCYNAATTTAAGCATAT(2012)
28SR0990
CCTTGGTCCGTGTTTCAAGAC
COILCO1490Folmer et al. (1994)Cesari et al. (2009)
GGTCAACAAATCATAAAGATATTGG

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Notes

Published as part of Zhang, Jing-Yu, Sun, Xue-Ling, Wang, Ning, Hao, Li, Ma, Cheng-Xue, Zhao, Na, Li, He- Ping, Zhao, Min & Yang, Sheng-Tao, 2024, Tardigrades in the alpine region of Northeast China with an integrative description of Crenubiotus liangshuiensis sp. nov., pp. 97 in Zootaxa 5492 (1) on page 97, DOI: 10.11646/zootaxa.5492.1.5, http://zenodo.org/record/13212067

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Journal article: 10.11646/zootaxa.5492.1.5 (DOI)
Journal article: urn:lsid:plazi.org:pub:FFDAFFF4867BFFBAFFEAE50FFFA95857 (LSID)
Journal article: http://publication.plazi.org/id/FFDAFFF4867BFFBAFFEAE50FFFA95857 (URL)
Journal article: http://zenodo.org/record/13212067 (URL)
Journal article: http://zoobank.org/336B4DA3-553E-4EAA-9277-272D386378DB (URL)

References

  • Medlin, L., Elwood, H. J., Stickel, S. & Sogin, M. L. (1988) The characterization of enzymatically amplified eukaryotic 16 S-like rRNA-coding regions. Gene, 71, 491 - 499. https: // doi. org / 10.1016 / 0378 - 1119 (88) 90066 - 2
  • Guidetti, R., Peluffo, J. R., Rocha, A. M., Cesari, M. & de Peluffo, M. C. M. (2013) The morphological and molecular analyses of a new South American urban tardigrade offer new insights on the biological meaning of the Macrobiotus hufelandi group of species (Tardigrada: Macrobiotidae). Journal of Natural History, 47, 2409 - 2426 https: // doi. org / 10.1080 / 00222933.2013.800610
  • Kiehl, E., Dastych, H., D'Haese, J. & Greven, H. (2007) The 18 S rDNA sequences support polyphyly of the Hypsibiidae (Eutardigrada). Journal of Limnology, 66, 21 - 25. https: // doi. org / 10.4081 / JLIMNOL. 2007. S 1.21
  • Mironov, S., Dabert, J. & Dabert, M. (2012) A new feather mite species of the genus Proctophyllodes Robin, 1877 (Astigmata: Proctophyllodidae) from the Long-tailed Tit Aegithalos caudatus (Passeriformes: Aegithalidae) - morphological description with DNA barcode data. Zootaxa, 3253 (1), 54 - 61. https: // doi. org / 10.11646 / zootaxa. 3253.1.2
  • Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294 - 299.
  • Cesari, M., Bertolani, R., Rebecchi, L. & Guidetti, R. (2009) DNA barcoding in Tardigrada: The first case study on Macrobiotus macrocalix Bertolani & Rebecchi 1993 (Eutardigrada, Macrobiotidae). Molecular Ecology Resources, 9, 699 - 706. https: // doi. org / 10.1111 / j. 1755 - 0998.2009.02538. x