Unicorn: Enhancing Single-Cell Hi-C Data with Blind Super-Resolution for 3D Genome Structure Reconstruction

In this study, we present ScUnicorn, a novel blind Super-Resolution framework for scHi-C enhancement. ScUnicorn employs an iterative degradation kernel optimization process, unlike traditional Super-resolution approaches, which rely on downsampling, predefined degradation ratios, or constant assumptions about the input data to reconstruct high-resolution interaction matrices. Hence, our approach more reliably preserves critical biological patterns and minimizes noise. Additionally, we propose 3DUnicorn, a maximum likelihood algorithm that leverages the enhanced scHi-C data to infer precise 3D chromosomal structures.