CRISPRme Version 2.1.7 Release Notes

Release Description

This release introduces CRISPRme v2.1.7, bringing improvements and bug fixes to off-target analysis, error tracing, and dataset compatibility. This update enhances performance, refines reporting accuracy and reproducibility, and expands support for exome-based variant datasets.

Key Features and Changes

🚀 New Features

• Support for Exome GNOMAD VCFs
  • Added compatibility for exome-based gnomAD variant call format (VCF) files, allowing for a broader range of genetic variant analyses.

🐛 Bug Fixes

• Fix 1: Corrected handling of indels in off-target reporting
  • Fixed an issue where off-targets containing indel variants were either missing or misreported, improving detection accuracy.
• Fix 2: Fixed the visualization of the Personal Card tab
  • Resolved inconsistencies in the GUI and website display of personal card off-targets, improving usability.
• Fix 3: Closed stream after creating graphical reports
  • Ensured proper resource management by closing open file streams post-report generation to prevent memory issues.

🛠 Enhancements

• Improved contiguous target merge logic
  • Enhanced merging logic to always report the leftmost off-target in target clusters, improving clarity in clustered results.
• Improved error tracing logic
  • Refined error tracking to provide more detailed and informative debugging messages.
• Upgraded Version available on DockerHub
  • The latest version of CRISPRme is now available for seamless deployment via Docker.

Upgrade Instructions

To upgrade to version 2.1.7, follow this steps:

1. Create a new environment (if using mamba or conda):

mamba create -n crisprme
mamba activate crisprme
mamba install crisprme=2.1.7

1. Docker: Pull the latest version from DockerHub:

docker pull pinellolab/crisprme:latest

Linked Issues

Closes #73, #74 related to this release.

This release further refines CRISPRme's capabilities, ensuring more reliable and accurate off-target analysis. We appreciate your feedback and contributions—thank you for supporting CRISPRme!

Full Changelog: https://github.com/pinellolab/CRISPRme/compare/v2.1.0...v2.1.7