Dataset for "Cell Painting PLUS: Expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles"

This data set includes all relevant source data (aggregated and processed profiles) as well as image analysis pipelines (Harmony and Cell Profiler software), KNIME pipelines and R code for data analysis and generation of plots for figures that support the key findings published in:

Authors and Affiliations

German Centre for the Protection of Laboratory Animals (Bf3R), German Federal Institute for Risk Assessment (BfR), Berlin, Germany

Elena von Coburg, Marlene Wedler, Jose M. Muino, Sebastian Dunst & Shu Liu

Department of Food Chemistry, University of Potsdam, Potsdam, Germany

Elena von Coburg

Institute of Biology, Free University of Berlin, Berlin, Germany

Marlene Wedler

Institute of Clinical Pharmacology and Toxicology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

Jose M. Muino

Screening Unit, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany

Christopher Wolff

Centre for Artificial Intelligence in Public Health Research, Robert Koch Institute, Berlin, Germany

Nils Körber

Contributions

S.L. and S.D. conceived the project and devised the research plan. E.C., M.W. performed the experiments. J.M.M., S.L., S.D., E.C. and M.W. conducted image and data analysis. N.K. established the image registration 4i stitcher software. C.W. generated compound plates and supported image and data analysis. E.C., S.L., and S.D. wrote the main manuscript text, and prepared figures and tables. All authors reviewed the manuscript.

Corresponding authors

Correspondence to sebastian.dunst@bfr.bund.de or shu.liu@bfr.bund.de.

Author notes

These authors contributed equally: Sebastian Dunst, Shu Liu.