3D Bioprinting of Collagen-based High-Resolution Internally Perfusable Scaffolds for Engineering Fully-biologic Tissue Systems (CAD Files)

These 3D part files were developed for "3D Bioprinting of Collagen-based High-Resolution Internally Perfusable Scaffolds for Engineering Fully-biologic Tissue Systems". There are the source files used in this primary research publication. All STLs are provided and detailed materials and methods can be found within the initial manuscript. 

All VAPOR bioreactors were 3D printed to Biomed Resin from Formlabs. All CHIPS were printed from collagen-based hydrogels and fibrin-based cellular bioinks.

Organ-on-a-chip and microfluidic systems have improved the translational relevance of in vitro systems; however, current manufacturing approaches impart limitations on materials selection, non-native mechanical properties, geometric complexity, and cell-driven remodeling into functional tissues. Here we 3D bioprint extracellular matrix (ECM) and cells into Collagen-based High-resolution Internally Perfusable Scaffolds (CHIPS) that integrate with a Vascular And Perfusion Organ-on-a-chip Reactor (VAPOR) to form a complete tissue engineering platform. We improve the fidelity of FRESH bioprinting to produce a range of CHIPS designs fabricated in a one-step process. CHIPS exhibit size-dependent permeability of perfused molecules into the surrounding scaffold to support cell viability and migration. Together CHIPS and VAPOR form a platform technology towards engineering full organ-scale function for disease modeling and cell replacement therapy.