Peroneutypa zhujiashanesis X. Y. Mao & Y. Q. Kang sp. nov.

Fig. 5

Etymology.

The epithet refers to the name of the location where the type specimen was collected, Zhujiashan National Forest Park.

Type.

China • Guizhou Province, Douyun City, Weng‘an County, Zhujiashan National Forest Park (107°38'35"E, 26°58'35"N), altitude 848 m, on branches of an unidentified plant, 14 February 2022. Xin Y Mao & Y. Q. Kang, ZJS 14 (Holotype GZAAS 24-0023; KUN-HKAS 133155; ex-type GZCC 24-0198). GenBank accession numbers (ITS: PP 852363; tub 2: PQ 301421).

Description.

Saprobic on decaying branches of an unknown tree. Sexual morph: Stromata 1–2.8 mm in diameter, immersed in the host surface, with necks conspicuously protruding through the bark, erumpent through an ostiolar canal, solitary to gregarious, 1–3 locules, mostly solitary, arranged irregularly, dark brown to black, glabrous, circular to irregular in shape, arranged irregularly, delimited by a black zone in host tissues. Ascomata (excluding neck) immersed in a stroma, dark brown to black, perithecia 550–910 μm high, 400–570 µm diam. (x ̄ = 790 × 520 μm, n = 10), black, single to aggregated, globose to sub-globose, each has an individual ostiole with a long neck. Ostiolar canals erumpent, smooth, 50–145 μm (x ̄ = 134.69 μm) in length, cylindrical, and curved at the apex. Peridium 24–42 μm (x ̄ = 32.73 μm) thick, composed of two layers, outer layer dark brown to black, cells thick-walled, texture angularis, inner layers hyaline, cells flattened, texture angularis. Paraphyses 3.5–6 μm (x ̄ = 5.4 μm, n = 20) wide, wider at the base, long, septate, smooth-walled, constricted at septa. Asci 23–31 × 3.5–7 μm (x ̄ = 27.5 × 5.5 μm, n = 30), unitunicate, 8 - spored, clavate, apically truncates, with a J- apical ring. Ascospore 3.5–5 × 1–1.5 μm (x ̄ = 4.2 × 1.3 μm, n = 30), overlapping, allantoid, subhyaline, smooth, aseptate, with 1–2 small guttules. Asexual morph: undetermined.

Culture characteristics.

Colonies grow fast on PDA, reach 60 mm in 1 week at 28 ° C, effuse towards the edge, from above at first white, becoming dirty white at the edge after 2 weeks, from below black at center, the rest white.

Additional specimens examined.

China • Guizhou Province, Anlong county, Xianheping National Forest Park (105°36'26"E, 24°58'39"N) altitude: 1298 m, on branches of an unidentified plant, 30 May 2022; Xin Y Mao & Y. Q. Kang, XHP 01 (Paratype GZAAS 24-0024; KUN-HKAS -133158; ex-paratype GZCC 24-0199). GenBank accession numbers (ITS: PP 852362; tub 2: PQ 301422).

Notes.

Phylogenetically, Peroneutypa zhujiashanesis is closely related to P. leucaenae. Morphologically, it also shares similarities with P. leucaenae in terms of ascomata size and the shape and size of paraphyses. However, P. zhujiashanesis can be distinguished from P. leucaenae by its smaller asci (23–31 × 3.5–7 μm vs. 30–37 × 3.8–4.5 μm) and longer ascospores (3.5–5 μm vs. 2.9–3.7 μm) (Samarakoon et al. 2022). Additionally, P. zhujiashanesis has smaller ostiolar necks (50–145 μm) and a J – apical ring, whereas P. leucaenae has longer ostiolar necks (275–350 μm) and an amyloid apical ring.

Morphologically, Peroneutypa zhujiashanesis is also like P. diminutiasca in ascospore size and presence of a J – subapical ring. However, P. diminutiasca differs by having smaller ascomata (147–218 μm in diameter), with longer ostiolar neck (average 193 μm vs 134.6 μm), and possessing 1–10 locules per ascomata (vs 1–3 loculate, mostly single) (Du et al. 2022).

Based on these morphological differences (Table 2) and phylogenetic evidence, we introduce our collection as a new species, Peroneutypa zhujiashanesis.