Aptamer-facilitated Protein Isolation from Cells

Protein isolation from cell lysate can represent a significant challenge. The most efficient way to purify a protein is through the use of target-specific affinity ligands. Development of such affinity ligands, in most cases requires the availability of the target protein in a pure form. Thus, isolating the protein from the cell lysate requires affinity ligands, while developing affinity ligands requires a pure protein. Aptamer-facilitated Protein Isolation from Cells (AptaPIC) resolves this loop problem. AptaPIC is a technique that allows the development of aptamers for a protein in a context of a cell lysate, without any prior purification steps, and the subsequent utilization of the developed aptamers for protein isolation from the cell lysate.