Published 2025 | Version v1
Dataset Open

Spatial Transcriptomics and Single Nucleus RNA Sequencing Reveal rAAV2- and rAAV9-Specific Transduction Signatures in the Mouse Liver

  • 1. ROR icon University of Bern
  • 2. ROR icon Roche (Switzerland)
  • 3. University of Heidelberg Bioquant

Description

You can find here the datasets used in the publication: 

Amberg, B. et al.  Spatial Transcriptomics and Single Nucleus RNA Sequencing Reveal rAAV2- and rAAV9-Specific Transduction Signatures in the Mouse Liver

This contains the raw Spatial Transcriptomics data, the TIF images used as spaceranger input, and intermediary andata objects that contrain the zones.
The scripts are available in Github: https://github.com/alberto-valdeolivas/ST_SN_AAV_MiceLiver



The results of the deconvolution can be found here.

The BAM files of the spatial transcriptomics data can be found here
- Male control animals
- Female control animals
- Male rAAV2-treated animals
- Female rAAV2-treated animals
- Male rAAV9-treated animals
- Female rAAV9-treated animals

The Fastq files of the spatial transcriptomics data can be found here 
- Male control animals part 1 and  part 2
- Female control animals part 1 and part 2
- Male rAAV2-treated control animals part 1 and part 2
- Female rAAV2-treated control animals part 1 and part 2
- Male rAAV9-treated control animals
- Female rAAV9-treated control animals part 1 and part 2

The single nucleus RNA sequencing (snRNA-seq)  can be found here
snRNA-seq cell ranger and cellbender output 
Fastq files of the snRNA-seq can be found here
- Male control animals animal 1, animal 2, animal 3
- Female control animals animal 1, animal 2, animal 3
- Male rAAV2-treated animals animal 1, animal 2, animal 3animal 4
- Female rAAV2-treated animals animal 1, animal 2, animal 3, animal 4
- Male rAAV9-treated animals animal 1, animal 2, animal 3, animal 4
- Female rAAV9-treated animals animal 1, animal 2 rep1, animal 2 rep2, animal 3animal 4

 

Files

SN275_B1_202.tif

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