GetGenome--Genomics for All
Published February 18, 2025 | Version v1
Annotation collection Open

Whole-genome sequence of vermi-compost isolated Bacillus strains with biocontrol and plant growth-promoting properties on Solanum lycopersicum seedling

Authors/Creators

  • 1. CONICET
  • 2. Universidad Nacional de Río Cuarto, FCEFQyN, Departamento de Biología Molecular, Río Cuarto, Córdoba, Argentina.
  • 3. CONICET, Universidad Nacional de Río Cuarto, Instituto de Biotecnología Ambiental y Salud, (INBIAS), Río Cuarto, Córdoba, Argentina

Description

Biological control agents are increasingly used to manage Fusarium infections. Specific microorganisms, such as various Bacillus species, show promise in suppressing Fusarium oxysporum f.sp. lycopersici (Fol), which causes Fusarium wilt in tomatoes. Here, the whole genome sequencing of Bacillus amyloliquefaciens 05JL, Bacillus amyloliquefaciens 07JL and Bacillus velezensis 11JL are announced. The genome of Bacillus amyloliquefaciens 05JL size is 4131480bp, distributed in 18 contigs with an N50 of 528543 and 46.13% GC. The genome of Bacillus amyloliquefaciens 07JL size is 4131480bp, distributed in 18 contigs with an N50 of 494614 and 46.12% GC. The genome of Bacillus amyloliquefaciens 11JL size is 4039844 bp, distributed in 11 contigs with an N50 of 999826 and 46.20% GC. These strains exhibit potent antifungal activity against Fusarium oxysporum both in vitro and in tomato plants. The analysis of these bacillus genomes may lead to gain insights of new enzymes and other biomolecules of interest for its application in the fields of agriculture as a biocontrol and plant growth-promoting microorganisms.

Files

WGS-BC-JL_Zenodo version final.pdf

Files (665.8 kB)

Name Size Download all
md5:42b320df8d20b2fc2a42cbca0c351e89
665.8 kB Preview Download

Additional details

References

  • [1] Medeot D, Sannazzaro A, Estrella MJ, Torres Tejerizo G, Contreras-Moreira B, Pistorio M, Jofré E. Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis. Sci Rep. 2023 Dec 13;13(1):22168. doi: 10.1038/s41598-023-49194-y. PMID: 38092837; PMCID: PMC10719345. [2] Bolger, A. M., Lohse, M., & Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics, 30(15), 2114–2120. [3] Bankevich, A., Nurk, S., Antipov, D., Gurevich, A. A., Dvorkin, M., Kulikov, A. S., Pevzner, P. A. (2012). SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing. Journal of Computational Biology, 19(5), 455–477. [4] Wick RR, Judd LM, Gorrie CL, Holt KE (2017) Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLOS Computational Biology 13(6): e1005595. [5] Blom J., Kreis J., Spänig S., Juhre T., Bertelli C., Ernst C., Goesmann A., EDGAR 2.0: an enhanced software platform for comparative gene content analyses, Nucleic Acids Res. 44 (2016) W22–W28, https://doi.org/10.1093/nar/gkw255. [6] Goris J., Konstantinidis K.T., Klappenbach J.A., Coenye T., Vandamme P., Tiedje J.M., DNA–DNA hybridization values and their relationship to whole genome sequence similarities, Int. J. Syst. Evol. Microbiol. 57 (2007) 81–91, https://doi.org/10.1099/ijs.0.64483-0.