Formulation And Evaluation of Ocimum Sanctum and Zingiber Officinale Loaded Liquid Dosage Formulation for Synergistic Anticoagulant Effect

Introduction: The use of Ocimum sanctum (holy basil, tulsi) from Lamiaceae family and Zingiberofficinale (ginger, adrakh) from Zingiberaceae is quite common in day to day life of Indian families.Ocimum sanctum (tulsi) from Lamiaceae family and Zingiberofficinale (ginger) from Zingiberaceae has been widely used for thousands of years in Ayurveda and Unani systems to cure or prevent a number of illnesses. The objective is to investigate, formulate as well as evaluate the synergistic effect of O. sanctum (Tulsi) and Z. officinale (ginger) by its succesive extraction. Coagulation activity of O. sanctum and Z. officinale was measured by in-vivo and in-vitro prothrombin time determination method on blood samples. Material And Method: The raw plants were subjected to successive extraction process followed by its in vitro and in vivo prothrombin time determination. The optimized extracts were combined and used for formulation and evaluation of liquid dosage formulation. Result: The extracts B2 and G3 were found to have best results. Conclusion: Finding led to the conclusion that O. sanctum leaves and Z. officinale rhizomes may act as an anticoagulant and thus, potentially may replace the current conventional anticoagulant drugs. Therefore, the work opens chances of new discoveries on future prospective. The investigation and development of new formulation in oral or parenteral route can be done for herbal anticoagulation therapy.