Enantioseparation of racemic compounds in capillary electrophoresis using cationic single isomer cyclodextrin

Capillary electrophoresis (CE) is a relatively new separation technique that provides rapid analysis with high efficiency and resolution due to the use of electric field and a variety of selective modes. Cyclodextrins are the most commonly utilized chiral selectors added into the background electrolyte to perform enantioseparation. This protocol describes an example of CE enantioseparation using a recently reported chiral selector mono-6-deoxy-6-(3R,4R-dihydroxypyrrolidine)-β-CD chloride (dhypy-CDCl).