Published February 4, 2025 | Version v1
Dataset Open

Source Data and protocols for White, et al 2025- The pyrethroid insecticide deltamethrin disrupts neuropeptide and monoamine signaling pathways in the gastrointestinal tract

Authors/Creators

  • 1. ROR icon Emory University School of Medicine

Contributors

Data collector:

Description

Here, we provide source data, a list of key resources, and manufacturer protocols used in the manuscript: https://doi.org/10.1101/2024.12.14.628386, which describes the toxicological outcomes of pyrethroid exposure in enteroendocrine cells and the gastroinestinal tract.

An excel spreadsheet containing the source data and statistical output, used in figure generation, and organized by figure panel, are included as the "SourceDataandStatistics" file.  A spreadsheet file of the raw CT values, and gene expression calcuations, for the indicated qPCR gene expression analysis is included in the "QPCRDataAnalysis" file.

RNAseq analysis for this project is further described in: https://doi.org/10.1101/2024.12.14.628386; which includes all alignment files, methods, and statistical outputs. In addition, maufacturer's protocols used in the library preparation (NEBNext) and sequencing (Cbot) are included here.

Metascape pathway analysis and differential gene expression source data, used in figure generation of the associted manuscript are included here as the indicated "DEGSourceData" and "MetascapePathwayAnalysis" files.

A Key Resource Table, containing identifiers for additional protocols, assays, and reagents, used in the study is also included.


 

 

Files

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Additional details

Related works

Is supplement to
Journal article: 10.1101/2024.12.14.628386 (DOI)
Is supplemented by
Dataset: 10.5281/zenodo.14775918 (DOI)
Dataset: PRJNA1218260 (Other)

Funding

National Institute of Environmental Health Sciences
Interaction of Pyrethroid Exposure and the Microbiome on Parkinson's Disease related Pathologies 1R01ES032440-01A1
Aligning Science Across Parkinson's
Role of enteroendocrine cells in the origin of Parkinson’s pathology ASAP-020527
National Institute of Environmental Health Sciences
HERCULES: Exposome Research Center; Pilot Grants P30ES019776
National Institute of Environmental Health Sciences
Graduate and Postdoctoral Training in Environmental Health Science and Toxicology T32ES012870