Supplemental Material for "The EPS-I exopolysaccharide transforms Ralstonia wilt pathogen biofilms into viscoelastic fluids for rapid dissemination in planta."

Explanation of Files

• cblaster output - RSSC eps cluster.zip - One of the evolutionary analyses for genomes containing clusters like the RSSC eps cluster
• RSSC Biofilm Mobility.zip - Quantitative Tilt Assay Image Set measuring "biofilm mobility" with its paired Cell Profiler image analysis pipeline
• NonRSSC Biofilm Mobility.zip - Full image set of biofilms grown either on a flat or angled surface to measure "biofilm mobility" of NonRSSC biofilms
• Colony Size and Population Density.zip - Quantitative Colony size image set with its paired Cell Profiler image analysis pipeline, as well as CFU/colony data sheets
• Individual Colony Mobility.zip - Qualitative image set of individual colonies grown on either a flat or tilted surface to visualize biofilm mobility
• Rheometry data.zip - Full rheology dataset with paired metadata for each sample measured: cfu/mL, and moisture content
• Growth curve.zip - Growth curve data in .xlsx format exported from Agilent Biotek Gen5 Software (with additional tabs for data labelling and organization)
• Plant Colonization Assay.zip - Excel spreadsheet with the cfu/g measurments for the in planta colonization assay
• Microfluidics Images.zip - Complete image sets of all microfluidics channels and trials, as well as a .pdf file outlining the process of quantifying the biofilm coverage
• Rheology Sample Examples - Image set of examples of colony biofilm samples used for rheology experiments
• Moisture content measurement repeat.zip - Moisture content measurements were repeated for GMI1000 wild type and GMI1000 ΔepsB to more directly measure differences between the strains
• eps_phylogenetic_synteny_analysis.zip - The input and output files for the Cluster Reconstruction and Phylogenetic Analysis Pipeline (CRAP)
• eps&nucAB cluster BlastP analysis.zip - This file contains the link to the kbase narrative which contains all of the search settings and results for figure 4B and S12