Characterisation and quantitation expression analysis of recombinant proteins in plant complex mixtures using nanoUPLC mass spectrometry

Identification of recombinant protein expressed in a total soluble protein (TSP) plant extract by mass spectrometry is desirable and necessary to accelerate further processing steps. Basically, the protocol consists of an initial TSP sample preparation and trypsin digestion prior preliminary characterization of recombinant proteins expressed in TSP samples of transgenic soybean seeds utilizing a nanoUPLC-MSe. As low as a 50 ug TSP sample can be effectively analyzed. Experimental data for the TSP extraction and sample preparation are discussed. The development of the process takes up to 3 days.