Therapeutic Potential Effect of Broccoli Extract Induction of Apoptosis and Inhibition of Migration in Human Hepg2 Cells

This study was undertaken to investigate the anticancer effects of broccoli extract against liver cancer in vitro and to elucidate the underlying molecular mechanisms. MTT assay was used to monitor the proliferation rate. DAPI staining was used for apoptosis. Wound healing assays were used to monitor cell migration and invasion. Change in the cell morphology by Ic50 value was demonstrated. Sulforaphane extract of broccoli decreased the viability of the liver cancer HepG2 cells and exhibited an IC50 of 50 µM. DAPI staining revealed that Sulforaphane triggered apoptotic death of HepG2 cells. The results indicated that Sulforaphane is bioactive and effectively inhibits the proliferation of cancer cell lines: HEPG2 human liver cells. Sulforaphane is a potential anticancer agent and may be considered a lead molecule in the development of hepatocellular chemotherapy

Keywords: Liver, apoptosis, migration, invasion, proliferation