Pancreatic islet transplantation to the renal subcapsule in mice

Mouse islet subcapsule transplantation is widely used in diabetes-related studies. Reliable and reproducible transplantation is essential to the success of those types of investigations. The present protocol presents detailed procedures on the mouse diabetic model and islet implantation. The blood glucose tracings under varied transplant circumstances are presented, covering syngeneic, allogeneic and xenogeneic islet transplantations. This protocol is straightforward and has been proven to be practical and reproducible.