Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension for analysis of repetitive DNA by flow cytometry

This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition.