Caffeic Acid Has Antiviral Activity against Ilhéus Virus In Vitro
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Saivish, Marielena Vogel1
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Pacca Mazaro, Carolina
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Gomes da Costa, Vivaldo
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de Lima Menezes, Gabriela
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da Silva, Roosevelt2
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Nebo, Liliane3
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Celestino Dutra da Silva, Gislaine
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Milhim, Bruno
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Da Silva Teixeira, Igor
- Henrique, Tiago1
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Mistrão, Natalia
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Miranda Hernandes, Victor4
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Zini, Nathalia
- de Carvalho, Ana Carolina5
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Fontoura, Marina Alves
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Rahal, Paula6
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Sacchetto, Lívia1
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Marques, Rafael Elias7
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Nogueira, Mauricio Lacerda8, 1
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1.
Faculdade de Medicina de São José do Rio Preto
- 2. Universidade Federal de Jataí
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3.
Universidade Federal de Goiás
- 4. Laboratório de virologia - LPV
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5.
Universidade Estadual de Campinas (UNICAMP)
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6.
Universidade Estadual Paulista (Unesp)
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7.
Brazilian Center for Research in Energy and Materials
- 8. University of Texas Medical Branch
Description
Ilhéus virus (ILHV) is a neglected mosquito-borne flavivirus. ILHV infection may lead to Ilhéus fever, an emerging febrile disease like dengue fever with the potential to evolve into a severe neurological disease characterized by meningoencephalitis; no specific treatments are available for this disease. This study assessed the antiviral properties of caffeic acid, an abundant component of plant-based food products that is also compatible with the socioeconomic limitations associated with this neglected infectious disease. The in vitro activity of caffeic acid on ILHV replication was investigated in Vero and A549 cell lines using plaque assays, quantitative RT-PCR, and immunofluorescence assays. We observed that 500 µM caffeic acid was virucidal against ILHV. Molecular docking indicated that caffeic acid might interact with an allosteric binding site on the envelope protein.
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Additional details
Identifiers
- PMID
- 36851709
- Other
- PMC9961518
Funding
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- 2020/12875-5
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- 2018/15083-2
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- 2013/21719-3
- National Institutes of Health
- The Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics (CREATE-NEO) U01AI151807
- Coordenação de Aperfeicoamento de Pessoal de Nível Superior
- #88881.506794/2020–01
- Universidade Estadual Paulista (Unesp)
- Edital PROPe 13/2022 4320
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- 2018/02993-0
- National Council for Scientific and Technological Development
- 141253/2019-3