Quantum Spin Resonance in Engineered Magneto-Sensitive Fluorescent Proteins Enables Multi-Modal Sensing in Living Cells

Three types of experiments are present in the dataset; compared to the associated article they are labelled using different terminology: magnetic field effect (MFE) measurements are labelled MARY (magnetically altered reaction yield) and optically detected magnetic resonance (ODMR) measurements are labelled RYDMR (reaction yield detected magnetic resonance) or RFE (radio field effect). Spectral data is stored in .mat (MATLAB) files which can be opened using the free python library scipy using the scipy.io.loadmat function. 

For RYDMR and MARY data, images are stored in multi-page tiff files (*.ome.tiff) which can be opened using python package tifffile, for instance:

from tifffile import TiffFile
def load_mary(basedir):
    datasets = glob.glob(basedir + "MARY_*.ome.tiff")
    sweeps = max([int(d.split("_")[-1].split(".")[0]) for d in datasets]) + 1
    all_imgs = []
    for i in range(sweeps):
        impath = basedir + f"MARY_{i}.ome.tiff"
        file = TiffFile(impath)
        imgs = [series.asarray() for series in file.series]
        imgs = np.stack(imgs)
        all_imgs.append(imgs)
    return all_imgs

Meta-data is stored in files named *config.json, and further experimental data (such as electromagnet control sequence or RF frequencies at each point) are stored in *data.npz files. In some cases pre-processed data is stored in *pl.npz files.

During the course of writing the manuscript, the chosen names to refer to the protein variants changed from the names used in the data meta data.

For data for Figures 1 and 2, all the MagLOV samples are MagLOV, and the numbering in the sample name refers to biological replicates (i.e. MagLOV 1, MagLOV 2, MagLOV 3 are all MagLOV).

For other figures where variants are compared, the conversion table is:

In some cases a dot is used to denote replicate experiments, e.g. MagLOV 14.1 is MagLOV 14, the .1 indicates we did multiple repeats and this was the first.

For assistance with loading the data, please do not hesistate to contact Gabriel Abrahams (gabriel.abrahams@eng.ox.ac.uk).

Version update: previous version had 5 second data for Figure 4, uploaded 10 second data as appears in manuscript.