Supplement data for the article "The impact of OTU sequence similarity threshold on diatom-based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)", in preparation

These are supplement data for the article "The impact of OTU sequence similarity threshold on diatom-based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)", in preparation

The folowing files are available:

• Supplement 1. Map of Mayotte with the sampling sites and the rivers.
• Supplement 2. rbcL primers, reaction mixture, and conditions used for the PCR of the 312-bp rbcL fragment. The information provided is for a single reaction with a final volume of 25µL.
• Supplement 3. The 20 fastq files containing the demultiplexed DNA reads.
• Supplement 4. Number of sequence reads for each sample before and after the trimming procedure.
• Supplement 5. The 20 OTU lists, corresponding to the 20 SSTs, including the number of DNA reads within the 90 samples and their assigned taxonomy.
• Supplement 6. Sampling site description with sample codes, names of rivers, year, number of raw DNA reads and GPS coordinates.
• Supplement 7. Values and summary statistics for the environmental variables.
• Supplement 8. The script used in Mothur for the bioinformatic analysis from trimming to the used OTU lists.