Published December 2, 2024 | Version v1

Genetic diversity and structure analysis of 'Ak Sakı' and 'Kara Sakı' apple cultivars growing in Erzincan/Türkiye

  • 1. Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Aydın Adnan Menderes University, Aydin, Türkiye
  • 2. Department of Nutrition and Dietetics, Faculty of Health Sciences, Erzincan Binali Yıldırım University, Erzincan, Türkiye
  • 3. Department of Medical Services and Techniques, Vocational School of Health Services, Selcuk University, Konya, Türkiye
  • 4. Bilkent University, Department of Molecular Biology and Genetics, Ankara, Türkiye
  • 5. Isparta Egirdir Fruit Research Institute, Isparta, Türkiye
  • 6. Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Institute of Biotechnology, Nitra, Slovakia

Description

In this study, ‘Ak Sakı’ and ‘Kara Sakı’ apple cultivars were collected from
different locations in Erzincan province, Türkiye, and genetic diversity
was determined using the Start Codon Targeted (SCoT) marker technique.
The SCoT marker technique was chosen because its gene targeting, long
primer, and high annealing temperature make it more effective than other
marker techniques. Using ten SCoT primers, 60 bands were obtained, and
42 of them were polymorphic. The polymorphism rate was determined to be
70%. The UPGMA (Unweighted Pair Group Method with Arithmetic mean)
dendrogram created using the PAUP 4.0b10 program consists of two clades.
The genetic distance between apple cultivars varies between 0.13462 and
0.45614. Principal Component Analysis (PCA) results were compatible with
the UPGMA dendrogram. With the SCoT marker technique, genetic diversity
among apple cultivars can be determined in a shorter time and with more
reliable results.
 

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Sevindik et al., 2025.pdf

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Identifiers

ISSN
2217-4605

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