Experimental dataset: "Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure"

This repository contains experimental data supporting our paper "Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure".

Original Publication:
Tah, I., Haertter, D., et al. (2024). Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure. PNAS (in press).
Preprint available at: https://doi.org/10.1101/2023.12.20.572544

Dataset Structure

amnioserosa_time_lapses/

This directory contains confocal microscopy time-lapse recordings of eCadherin-labeled Drosophila melanogaster embryos during dorsal closure:

• Imaging parameters:
  • Frame interval: 15 seconds
  • Pixel size: 0.158 µm
• Data files:
  • *_data.p: Morphometric features of individual amnioserosa cells during closure
  • *_junctions.p: Morphometric features of individual cell-cell adherens junctions
  • read_filter_data.py: Python script demonstrating data reading and filtering procedures

junction_laser_cuts/

This directory contains high-speed recordings of UV-laser junction ablation experiments:

• Imaging parameters:
  • Frame rate: 5 Hz
  • Pixel size: 0.364 µm
• Content: Time series of eCadherin-labeled embryos before, during, and after precise UV-laser severing of individual junctions

data_figures/

This directory contains processed data and analysis results presented in Figures 1-3 of the main manuscript. The data are organized by figure number and include all measurements and simulation results used to generate the plots shown in these figures.

For detailed experimental methods and protocols, please refer to the published paper.