Published November 6, 2024 | Version v1

Gradual Pressure Error in an HPLC-HRMS Measurement Run

Description

This dataset contains files of a reverse-phase HPLC-HRMS orbitrap measurement run during which the pump pressure gradually got more unstable. While a run was being recorded, the pump for the organic eluent ceased functioning slowly. This resulted in a high amount of new signals appearing in the form of "stripes" at specific retention times.

It is intended as a control group for assigned data quality scores, with the reasoning that a highly unstable pressure in the chromatography will lead to more purely noise-induced signals. The relevant pressure profiles are also included. The mzML files were produced from the raw data using MSconvert and only contain the MS1 spectra.

The last file in the series (20240805_AA_DK_Ibu_pH6_1min_20240808161549) remains incomplete since the instrument shut off halfway through the measurement.

Files

Blank_01_Pump_Pressure.txt

Files (5.9 GB)

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