Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection
Creators
- Enebral-Romero, Estefanía (Researcher)
- García-Fernandez, Daniel (Researcher)
- Gutierrez-Galvez, Laura (Researcher)
- Lopez-Diego, David (Researcher)
- Luna, Monica (Researcher)
- García-Martín, Adrian (Researcher)
- Salagre, Elena (Researcher)
- Michel, Enrique G. (Researcher)
- Torres, Inigo (Researcher)
- Zamora, Felix (Researcher)
- García-Mendiola, Tania (Researcher)
- Lorenzo, Encarnacion (Researcher)
Description
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL 1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens.
Files
1-s2.0-S0956566324005050-main.pdf
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Additional details
Funding
Dates
- Accepted
-
2024-06-12