Proteomic Analysis of Skin Punch Biopsies from a Parkinson's Disease Patient Cohort: A PPMI Pilot Study

To facilitate a larger proteomic study of human skin from Parkinson's disease patient cohorts, a spectral library of the human skin proteome was generated through mass spectrometry (MS) analysis. For this purpose, twenty-two 3mm skin tissue punch biopsies from the C8 paravertebral region collected from a single Parkinson's disease patient cohort were analysed by data-independent acquisition mass spectrometry (DIA-MS) proteomics.

The protocol for sample preparation, DIA-MS proteomics and data analysis can be found here: https://www.protocols.io/view/proteomics-sample-preparation-of-human-skin-punch-14egn9e46l5d/v1

Raw mass spectrometry data generated from the analysis were deposited to the PRIDE database and can be accessed here: https://www.ebi.ac.uk/pride/archive/projects/PXD060089 or searching using project identifier: PXD060089

This data and protocol were generated as part of a pilot study in collaboration with the Michael J. Fox Foundation. The biospecimens were obtained from the Parkinson’s Progression Marker Initiative (PPMI) (RRID:SCR_006431). For up-to-date information on the study, visit www.ppmi-info.org.