Published June 11, 2025
| Version v1
Dataset
Open
Dataset for Article: Drebrin upregulation regulates astrocyte polarization and supports tissue recovery after spinal cord injury in mice
Authors/Creators
Files
Figure 1.TIF
Files
(11.1 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:d36fd770278d14582e3a935841d1e4d0
|
14.1 kB | Download |
|
md5:07c756a24531fb235167f8fd9c480bcb
|
688.5 kB | Download |
|
md5:747a4995d8350cc601a6b94020270c68
|
36.1 MB | Preview Download |
|
md5:ee98e24e5af32636ceb6c6f9937ff7f0
|
5.7 MB | Preview Download |
|
md5:68e83f7ee69d4bb8cb930d8b2e18fb2f
|
24.4 MB | Preview Download |
|
md5:ee24d3fbef02d18f53c02e21f838e96c
|
3.4 MB | Preview Download |
|
md5:c143397735176c074181c365b2bd736c
|
66.5 MB | Preview Download |
|
md5:08a43e89342cfabc37b7e3c7d3d98644
|
66.1 MB | Preview Download |
|
md5:eeaffe91ba19f42c4dba0243aff79e19
|
12.2 MB | Preview Download |
|
md5:b2c09ca10978d91c8e80453197480407
|
10.6 GB | Download |
|
md5:1979f0c733c5c27215c389aea1085ffb
|
43.9 MB | Preview Download |
|
md5:548849546d6ed1d0c83b36d541ea2a41
|
44.6 MB | Preview Download |
|
md5:7c8815a2bd7c293b6588fbfc391ca674
|
22.5 MB | Preview Download |
|
md5:da18dc1ce0d6ec59d84c477ef1d08e1e
|
28.0 MB | Preview Download |
|
md5:b423eccee5b894951c6cdfc0847ef24e
|
2.3 MB | Preview Download |
|
md5:38d5591a4e83e943ece08078b9b37f5c
|
41.5 MB | Preview Download |
|
md5:573b64739f3797a342fd02312a13206e
|
46.6 MB | Preview Download |
|
md5:3966542a8d4a269ae0379db5bbac628f
|
5.8 MB | Preview Download |
Additional details
Identifiers
Related works
- Is supplement to
- Journal article: 10.1002/glia.70048 (DOI)
Funding
References
- Ahuja, C. S., Wilson, J. R., Nori, S., Kotter, M. R. N., Druschel, C., Curt, A., & Fehlings, M. G. (2017). Traumatic spinal cord injury. Nature Reviews Disease Primers, 3(1), 17018. https://doi.org/10.1038/nrdp.2017.18 Anderson, M. A., Burda, J. E., Ren, Y., Ao, Y., O'Shea, T. M., Kawaguchi, R., Coppola, G., Khakh, B. S., Deming, T. J., & Sofroniew, M. V. (2016). Astrocyte scar formation aids central nervous system axon regeneration. Nature, 532(7598), 195–200. https://doi.org/10.1038/nature17623 Aoki, C., Sekino, Y., Hanamura, K., Fujisawa, S., Mahadomrongkul, V., Ren, Y., & Shirao, T. (2005). Drebrin A is a postsynaptic protein that localizes in vivo to the submembranous surface of dendritic sites forming excitatory synapses. Journal of Comparative Neurology, 483(4), 383–402. https://doi.org/10.1002/cne.20449 Attwell, C. L., Van Zwieten, M., Verhaagen, J., & Mason, M. R. J. (2018). The Dorsal Column Lesion Model of Spinal Cord Injury and Its Use in Deciphering the Neuron‐Intrinsic Injury Response. Developmental Neurobiology, 78(10), 926–951. https://doi.org/10.1002/dneu.22601 Basso, D. M., Fisher, L. C., Anderson, A. J., Jakeman, L. B., Mctigue, D. M., & Popovich, P. G. (2006). Basso Mouse Scale for Locomotion Detects Differences in Recovery after Spinal Cord Injury in Five Common Mouse Strains. Journal of Neurotrauma, 23(5), 635–659. https://doi.org/10.1089/neu.2006.23.635 Beck, K. D., Nguyen, H. X., Galvan, M. D., Salazar, D. L., Woodruff, T. M., & Anderson, A. J. (2010). Quantitative analysis of cellular inflammation after traumatic spinal cord injury: Evidence for a multiphasic inflammatory response in the acute to chronic environment. Brain, 133(2), 433–447. https://doi.org/10.1093/brain/awp322 Bellver-Landete, V., Bretheau, F., Mailhot, B., Vallières, N., Lessard, M., Janelle, M.-E., Vernoux, N., Tremblay, M.-È., Fuehrmann, T., Shoichet, M. S., & Lacroix, S. (2019). Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury. Nature Communications, 10(1), 518. https://doi.org/10.1038/s41467-019-08446-0 Bradbury, E. J., & Burnside, E. R. (2019). Moving beyond the glial scar for spinal cord repair. Nature Communications, 10(3879). https://doi.org/10.1038/s41467-019-11707-7 Burda, J. E., Bernstein, A. M., & Sofroniew, M. V. (2016). Astrocyte roles in traumatic brain injury. Experimental Neurology. Chung, J., Franklin, J. F., & Lee, H. J. (2019). Central expression of synaptophysin and synaptoporin in nociceptive afferent subtypes in the dorsal horn. Scientific Reports, 9(1), 4273. https://doi.org/10.1038/s41598-019-40967-y Crowley, S. T., Fukushima, Y., Uchida, S., Kataoka, K., & Itaka, K. (2019). Enhancement of Motor Function Recovery after Spinal Cord Injury in Mice by Delivery of Brain-Derived Neurotrophic Factor mRNA. Molecular Therapy - Nucleic Acids, 17, 465–476. https://doi.org/10.1016/j.omtn.2019.06.016 David, S., & Kroner, A. (2011). Repertoire of microglial and macrophage responses after spinal cord injury. Nature Reviews Neuroscience, 12(7), 388–399. https://doi.org/10.1038/nrn3053 Deacon, R. M. J. (2013). Measuring Motor Coordination in Mice. Journal of Visualized Experiments, 75, 2609. https://doi.org/10.3791/2609 Hellenbrand, D. J., Quinn, C. M., Piper, Z. J., Morehouse, C. N., Fixel, J. A., & Hanna, A. S. (2021). Inflammation after spinal cord injury: A review of the critical timeline of signaling cues and cellular infiltration. Journal of Neuroinflammation, 18(1), 284. https://doi.org/10.1186/s12974-021-02337-2 Heneka, M. T., & Feinstein, D. L. (2001). Expression and function of inducible nitric oxide synthase in neurons. Journal of Neuroimmunology, 114(1–2), 8–18. https://doi.org/10.1016/S0165-5728(01)00246-6 Hill, R. L., Zhang, Y. P., Burke, D. A., Devries, W. H., Zhang, Y., Magnuson, D. S. K., Whittemore, S. R., & Shields, C. B. (2009). Anatomical and functional outcomes following a precise, graded, dorsal laceration spinal cord injury in C57BL/6 mice. Journal of Neurotrauma, 26(1), 1–15. https://doi.org/10.1089/neu.2008.0543 Janz, R., Südhof, T. C., Hammer, R. E., Unni, V., Siegelbaum, S. A., & Bolshakov, V. Y. (1999). Essential Roles in Synaptic Plasticity for Synaptogyrin I and Synaptophysin I. Neuron, 24(3), 687–700. https://doi.org/10.1016/S0896-6273(00)81122-8 Kajita, Y., Kojima, N., & Shirao, T. (2024). A lack of drebrin causes olfactory impairment. Brain and Behavior, 14(1), e3354. https://doi.org/10.1002/brb3.3354 Kreis, P., Gallrein, C., Rojas-Puente, E., Mack, T. G. A., Kroon, C., Dinkel, V., Willmes, C., Murk, K., tom-Dieck, S., Schuman, E. M., Kirstein, J., & Eickholt, B. J. (2019). ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans. Nature Communications, 10(1), 486. https://doi.org/10.1038/s41467-019-08420-w Mantovani, A., Sica, A., Sozzani, S., Allavena, P., Vecchi, A., & Locati, M. (2004). The chemokine system in diverse forms of macrophage activation and polarization. Trends in Immunology, 25(12), 677–686. https://doi.org/10.1016/j.it.2004.09.015 Masliah, E., Fagan, A. M., Terry, R. D., DeTeresa, R., Mallory, M., & Gage, F. H. (1991). Reactive Synaptogenesis Assessed by Synaptophysin lmmunoreactivity Is Associated with GAP-43 in the Dentate Gyrus of the Adult Rat. Experimental Neurology, 113(2), 131–142. https://doi.org/10.1016/0014-4886(91)90169-D Metz, G. A., & Whishaw, I. Q. (2002). Cortical and subcortical lesions impair skilled walking in the ladder rung walking test: A new task to evaluate fore- and hindlimb stepping, placing, and co-ordination. Journal of Neuroscience Methods, 115(2), 169–179. https://doi.org/10.1016/S0165-0270(02)00012-2 Metz, G. A., & Whishaw, I. Q. (2009). The Ladder Rung Walking Task: A Scoring System and its Practical Application. Journal of Visualized Experiments, 28, 1204. https://doi.org/10.3791/1204 Ohsawa, K., Imai, Y., Sasaki, Y., & Kohsaka, S. (2004). Microglia/macrophage‐specific protein Iba1 binds to fimbrin and enhances its actin‐bundling activity. Journal of Neurochemistry, 88(4), 844–856. https://doi.org/10.1046/j.1471-4159.2003.02213.x Okada, S., Nakamura, M., Katoh, H., Miyao, T., Shimazaki, T., Ishii, K., Yamane, J., Yoshimura, A., Iwamoto, Y., Toyama, Y., & Okano, H. (2006). Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury. Nature Medicine, 12(7), 829–834. https://doi.org/10.1038/nm1425 O'Shea, T. M., Burda, J. E., & Sofroniew, M. V. (2017). Cell biology of spinal cord injury and repair. Journal of Clinical Investigation, 127(9), 3259–3270. https://doi.org/10.1172/JCI90608 Paolicelli, R. C., Sierra, A., Stevens, B., Tremblay, M.-E., Aguzzi, A., Ajami, B., Amit, I., Audinat, E., Bechmann, I., Bennett, M., Bennett, F., Bessis, A., Biber, K., Bilbo, S., Blurton-Jones, M., Boddeke, E., Brites, D., Brône, B., Brown, G. C., … Wyss-Coray, T. (2022). Microglia states and nomenclature: A field at its crossroads. Neuron, 110(21), 3458–3483. https://doi.org/10.1016/j.neuron.2022.10.020 Riek-Burchardt, M., Henrich-Noack, P., Metz, G. A., & Reymann, K. G. (2004). Detection of chronic sensorimotor impairments in the ladder rung walking task in rats with endothelin-1-induced mild focal ischemia. Journal of Neuroscience Methods, 137(2), 227–233. https://doi.org/10.1016/j.jneumeth.2004.02.012 Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., & Cardona, A. (2012). Fiji: An open-source platform for biological-image analysis. Nature Methods, 9(7), 676–682. https://doi.org/10.1038/nmeth.2019 Schiweck, J., Murk, K., & Eickholt, B. J. (2018). Important Shapeshifter: Mechanisms Allowing Astrocytes to Respond to the Changing Nervous System During Development, Injury and Disease. Frontiers in Cellular Neuroscience, 12. https://doi.org/10.3389/fncel.2018.00261 Schiweck, J., Murk, K., Ledderose, J., Münster-Wandowski, A., Ornaghi, M., Vida, I., & Eickholt, B. J. (2021). Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking. Nature Communications, 12(1), 1490. https://doi.org/10.1038/s41467-021-21662-x Wang, X., Cao, K., Sun, X., Chen, Y., Duan, Z., Sun, L., Guo, L., Bai, P., Sun, D., Fan, J., He, X., Young, W., & Ren, Y. (2015). Macrophages in spinal cord injury: Phenotypic and functional change from exposure to myelin debris. Glia, 63(4), 635–651. https://doi.org/10.1002/glia.22774 Wang, X.-X., Li, Z.-H., Du, H.-Y., Liu, W.-B., Zhang, C.-J., Xu, X., Ke, H., Peng, R., Yang, D.-G., Li, J.-J., & Gao, F. (2024). The role of foam cells in spinal cord injury: Challenges and opportunities for intervention. Frontiers in Immunology, 15, 1368203. https://doi.org/10.3389/fimmu.2024.1368203 Wanner, I. B., Anderson, M. A., Song, B., Levine, J., Fernandez, A., Gray-Thompson, Z., Ao, Y., & Sofroniew, M. V. (2013). Glial Scar Borders Are Formed by Newly Proliferated, Elongated Astrocytes That Interact to Corral Inflammatory and Fibrotic Cells via STAT3-Dependent Mechanisms after Spinal Cord Injury. Journal of Neuroscience, 33(31), 12870–12886. https://doi.org/10.1523/JNEUROSCI.2121-13.2013 Willmes, C. G., Mack, T. G. A., Ledderose, J., Schmitz, D., Wozny, C., & Eickholt, B. J. (2017). Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin. Scientific Reports, 7(1), 42652. https://doi.org/10.1038/srep42652