Published November 3, 2023 | Version v1
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Table 2 in A revised taxonomy and phylogeny of opalinids (Stramenopiles: Opalinata) inferred from the analysis of complete nuclear ribosomal DNA genes

  • 1. State Key Laboratory of Freshwater Ecology and Biotechnology * and Key Laboratory of Aquaculture Disease Control * Ministry of Agriculture * Institute of Hydrobiology * Chinese Academy of Sciences * Wuhan 430072 * China
  • 2. State Key Laboratory of Freshwater Ecology and Biotechnology * and Key Laboratory of Aquaculture Disease Control * Ministry of Agriculture * Institute of Hydrobiology * Chinese Academy of Sciences * Wuhan 430072 * China & College of Advanced Agricultural Sciences * University of Chinese Academy of Sciences * Beijing 100049 * China
  • 3. Departamento de Microbiología y Parasitología * Facultad de Farmacia * Universidad Complutense de Madrid * Plaza Ramón y Cajal s / n * Madrid 28040 * Spain

Description

Table 2. Primers used for PCRs and sequencing (F* forward; R* reverse).

PrimerSequenceReference
MedlinA (F)5 ʹ -AACCTGGTTGATCCTGCCAGT-3%Medlin et al. (1988)
MedlinB (R)5 ʹ -TGATCCTTCTGCAGGTTCACCTAC-3%Medlin et al. (1988)
SSU-LSU-F (F)5 ʹ -TCGCACCTACCGATTGGATG-3%This study
SSU-LSU-R (R)5 ʹ -GAAAAGATAACTCTTCCTAG-3%This study
M28S-F (F)5 ʹ -TAGCCCTGAAAATGGATGGCGCT-3%This study
28S-4R (R)5 ʹ -TTCTGACTTAGAGGCGTTCAG-3%Moreira et al. (2007)

Notes

Published as part of Li, Ming, Hu, Guangran, Zhao, Weishan, Zou, Hong, Li, Wenxiang, Wu, Shangong, Wang, Guitang & Ponce-Gordo, Francisco, 2024, A revised taxonomy and phylogeny of opalinids (Stramenopiles: Opalinata) inferred from the analysis of complete nuclear ribosomal DNA genes, pp. 271 in Zoological Journal of the Linnean Society 201 (2) on page 271, DOI: 10.1093/zoolinnean/zlad150, http://zenodo.org/record/13220219

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Related works

Is part of
Journal article: 10.1093/zoolinnean/zlad150 (DOI)
Journal article: urn:lsid:plazi.org:pub:FFF4FFFA75649466F0042747FFB36E6E (LSID)
Journal article: http://zenodo.org/record/13220219 (URL)
Journal article: http://publication.plazi.org/id/FFF4FFFA75649466F0042747FFB36E6E (URL)
Journal article: http://zoobank.org/672E17B-149D-466C-9A6E-26C6DD65A7B6Corresponding (URL)

References

  • Medlin LK * Elwood HJ * Stickel S et al. The characterization of enzymatically amplified 16 S-like rRNA coding regions. Gene 1988; 71: 491 - 9.
  • Moreira D * von der Heyden S * Bass D et al. Global eukaryote phylogeny: combined small- and large-subunit ribosomal DNA trees support monophyly of Rhizaria * Retaria and Excavata. Molecular Phylogenetics and Evolution 2007; 44: 255 - 66.