Published January 31, 2018 | Version v1
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Antibiotic Resistance Patterns of Enterococcus faecalis and E. faecium Isolated from Infected Wounds. Incidence of High –level Resistance to Vancomycin and Aminoglycosides

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A total of fifty seven isolates of Entercoccus faecalis (N=42) and E. faecium (N=15) from infected wounds were screened for their resistance against 17 antibiotics including vancomycin. E. faecalis isolates demonstrated absolute resistance (100%) against eight antibiotics: chloramphenicol, nalidixic acid, gentamicin, streptomycin, tetracyclin, erythromycin, penicillin G and lincomycin. While isolates of E. faecium revealed absolute resistance (100%) to only three antibiotics: chloramphenicol, nalidixic acid, and lincomycin, yet they exhibited 80% resistance against gentamicin and streptomycin. Higher percentage of resistance against vancomycin was shown by E. faecium (53.3%) as compared to Ent. faecalis (47.6%). E. faecalis and E. faecium isolates were involved in 5 and 2 antibiotic resistance patterns (ARPs) respectively. Of the two species; 24.5% isolates (N=11, 26.2% E. faecalis and N=3, 20% E. faecium ) were included in one ARP demonstrating resistance to all antibiotics under study.

High-level resistance to glycopeptides (HLRG) vancomycin (64 µg/ml) was higher among isolates of E. faecium (50%) as compared to E. faecalis (30%). Whereas high-level resistance to aminoglycosides (HLRA): gentamicin (500 µg/ml) and streptomycin (>1000 µg/ml) was higher among E. faecalis against gentamicin (80% VS 62.5%) and almost similar incidence was reported by the two species against streptomycin (60% and 62.5% respectively). Emergence of varied ARPs and high-level resistance to vancomycin and aminoglycosides among E. faecalis and E. faecium isolates will reduce to a great extent the therapeutic options against enterococcal infections. Hence, we emphasizes on the importance of performing susceptibility testing on all clinically significant isolates.

 

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