NFDI4Bioimage Calendar 2024 October; original image

Published September 25, 2024 | Version v1

Image Open

1. Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases

Raw microscopy image from the NFDI4Bioimage calendar October 2024.

Technical info

Image Metadata (using REMBI template):

Study
Study description	Creation of a beautiful image for an image exhibition
General dataset info	Christian Jüngst (Acquisition), Working group of Prof. Matteo Bergami (Sample Preparation)
Study Component
Imaging method	Multiphoton microscopy
Biosample
Biological entity	Nerve cells
Organism	Mus musculus
Organism part	Dentate gyrus of the hippocampus
Specimen
Preparation method	Tissue section of a mouse brain
Signal mechanism	Fluorescent proteins
Channel 1 - content	EYFP
Channel 1 - biological entity	Thy1
Image Acquisition
Instrument attributes	TCS MP-OPO, Leica Microsystems. Upright Multiphoton microscope with a 25X water immersion objective
Image acquisition parameters	Acquisition of a z-stack with 173 steps in an interval of 2 µm
Image Data
Type	Maximum projection with color coded depth
Dimension extents	x: 1024, y: 1024, z:173
Size description	442.86 µm x442.86 µm x 343.79 µm
Pixel size description	0.433 µm x 0.433 µm x 2 µm
Image processing method	A Median filter was used to smooth the image and pixel amount was increased to print it out for the image exhibition

Name	Size	Download all
3.lif md5:42d2e0bc4cc4a23e3fe10dedb2ea2797	362.9 MB	Download