Neutrophil-chemoattractant CXCL5 regulates lung barrier permeability in acute lung injury

Online supplementary data including the seurat objects used in scRNA analysis 

AUTHORS:

Sarah Berger^1*, Cengiz Goekeri^1*, Peter Pennitz^1*, Birgitt Gutbier¹, Laura Michalick^2,3, Karen Hoffmann¹, Elena Lopez-Rodriguez⁴, Vladimir Gluhovic⁴, Sandra-Maria Wienhold¹, Ulrike Behrendt¹, Alexander Taylor¹, Kristina Dietert^5,6, Holger Kirsten⁷, Sandra Kunder⁶, Kristina Mueller^7,14, Torsten Hain⁸, Sarah M. Volkers¹, Sebastian Weis^9,10,11, Achim D. Gruber⁶, Leif E. Sander^1,12, Wolfgang M. Kuebler^2,3, CAPSyS study group¹³, Martin Witzenrath^1,12 and Geraldine Nouailles^1,12#

¹ Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Infectious Diseases, Respiratory Medicine and Critical Care, Berlin, Germany.

² Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Physiology, Berlin, Germany.

³ DZHK (German Centre for Cardiovascular Research), partner site Berlin, Berlin, Germany

⁴ Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Functional Anatomy, Berlin, Germany.

⁵ Veterinary Centre for Resistance Research, Freie Universität Berlin, Berlin, Germany.

⁶ Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany

⁷ Institute for Medical Informatics, Statistics, and Epidemiology, Universität Leipzig, Leipzig, Germany

⁸ Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus-Liebig University Giessen, Giessen, Germany.

⁹ Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital,

Friedrich-Schiller-University Jena, Germany

¹⁰ Institute for Infectious Disease and Infection Control, Jena University Hospital, Friedrich-Schiller-University Jena, Jena, Germany

¹¹ Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany

¹² German Center for Lung Research (DZL), Berlin, Germany

¹³ members of the CAPSyS study group: Sven Gläser, Henning Kahnert, Felix Rosenow, Peter Ahnert, Markus Scholz, Julio Vera-González, Bernd Thomas Schmeck, Markus Löffler, Uwe Völker, Sven Hammerschmidt, Michael Bauer, Michael Kiehntopf, Trinad Chakraborty, Petra Creutz, Aline leClaire, Agata Mikolajewska, Markus Vogtmann, Michaela Niebank, Carmen Garcia, Martin Witzenrath, Norbert Suttorp

¹⁴ Center for Scalable Data Analytics and Artificial Intelligence (ScaDS.AI), Leipzig University, Germany

* S.B., C.G. and P.P. contributed equally to this work;

^# corresponding author: Dr.-Ing. Geraldine Nouailles, Charitéplatz 1, 10117 Berlin, Germany, Email: geraldine.nouailles@charite.de

Support statement:

This research was supported by the German Federal Ministry of Education and Research, Germany (BMBF) grants e:Med CAPSyS - 01ZX1604B, e:Med SYMPATH - 01ZX1906A and MAPVAP - 01KI2124, by the German Center for Lung Research (Deutsches Zentrum für Lungenforschung, DZL) grants PROGRESS - 82DZLJ19B1 and funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project ID 431232613 – SFB 1449 and project ID 114933180 – SFB-TR84.

File description:

s565_1_seurat_scRNA_zenodo.rds

scRNA Seurat file from 10xV3, filtered and preprocessed, used for differential expression analysis and ligand–receptor interactions analysis.

Lung_pool1_L1.raw_feature_bc_matrix.h5 
Lung_pool1_L2.raw_feature_bc_matrix.h5
Lung_pool2_L1.raw_feature_bc_matrix.h5
Lung_pool2_L2.raw_feature_bc_matrix.h5 
Raw unfiltered h5 scRNA files from 10xV3 created by Cell Ranger

ST_Seu_Cxcl5_final.rds

spatial single cell Seurat file from 10x Visium Spatial Gene Expression slides, filtered and preprocessed, used in spatial single cell analysis