Published September 4, 2024 | Version v1
Dataset Open

Microbiota study IgG4-RD AG Chang

Authors/Creators

  • 1. German Rheumatology Research Centre, Berlin – A Leibniz Institute, Berlin, Germany
  • 2. Department for Cytometry, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany
  • 3. Department of Rheumatology, Campus Charité Mitte, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany

Description

We have investigated an IgG4-RD (IgG4-RD) cohort by our multi-parameter microbiota flow cytometry approach to characterise the microbiota on single-cell level for attributes of the disease. The microbiota is isolated from stool samples and stained according to the published protocol for (a) host immunoglobulins IgA1, IgA2, IgM, IgG and (b) agglutinin binding to mannose, galactose or N-Acetyl-glucosamine surface sugar moieties. For all samples we also determined the microbiome composition by 16S rRNA (V3-V4) sequencing on the illumina MiSeq platform. We provide the raw .fcs and FASTQ files of 40 IgG4-RD patients.

 

For comparison we additionally analysed 36 healthy donors.

All .fcs files were generated on BD Influx®.

 

The metadata is collected in the provided meta.csv.

The staining parameters are summarized in provided panel.csv.

Files

Fastq_IgG4.zip

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Additional details

Related works

Is derived from
Book chapter: 10.1016/bs.mcb.2024.02.023 (DOI)
Is variant form of
Publication: 10.1002/eji.202250337 (DOI)