3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES

Author: University of Nottingham Medical School Division of Histopathy ### METHOD: 1. Wash glass slides in detergent for 30 minutes. - Wash glass slides in running tap water for 30 minutes. - Wash glass slides in distilled water 2x5 minutes. - Wash glass slides in 95% alcohol 2x5 minutes. - Air dry for 10 minutes. - Immerse slides in a freshly prepared 2% solution for 3-aminopropyltriethoxysilane in dry acetone for 5 seconds. - Wash briefly in distilled water twice. - Dry overnight at 42C and store at room temperature. - *Note: 300mls of silane solution is sufficient to do 200 slides. Treated slides can be kept indefinitely*.