Published December 30, 2014 | Version v1
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EPON resin mixture for transmission electron microscopy

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  • 1. ScientificProtocols.org

Description

### For Epon WPE 153: ![Table 1](http://i.imgur.com/3JSUZme.png "Table 1") Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years. ### For use: 1. Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic. - Mix equal volumes of A and B together. - Then add 0.1 ml of DMP-30 for each 5 ml of plastic. - Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.

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