EVALUATION OF ANTIDIABETIC ACTIVITY OF BILE FROM GALLUS GALLUSDOMESTICUS

Diabetes mellitus is a chronic condition characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The current study aimed at evaluating the antidiabetic activity of bile from Gallus gallusdomesticus. The zoochemical analysis revealed the presence of steroids. The invitro antioxidant activity of the HMEB and in vitroantiinflammatory activity of HMEB was evaluated. The invitroantidiabetic activity was investigated by α-Amylase inhibition assay, and glucose uptake assay. The gene expression study was carried out for GLUT-4 and PPAR-ꝩ was compared with the housekeeping gene β-actin. The animal toxicity study was performed as per OECD test guidelines 423 limit test The OGTT was conducted in normal rats and observed significant blood glucose lowering with HMEB (high dose). Thus hydro-methanolic extract of bile from Gallus gallusdomesticus could be developed as a potential drug candidate for the treatment of diabetes mellitus.