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Published August 30, 2024 | Version CC-BY-NC-ND 4.0
Journal article Open

Overview of Analytical Methods for Characterizing the Charge Heterogeneity of Antibody-Drug Conjugates

Creators

  • 1. Department of Medicinal Chemistry and Quality Control, Faculty of Pharmacy, University of Tishreen, Latakia, Syria.

Description

Abstract: Antibody-drug conjugates (ADCs) are ideal candidates for selective therapy. ADCs display considerable heterogeneity resulting from various modifications. One of the molecular characteristics of ADCs is charge heterogeneity. Several analytical methods could be used to characterize the charge heterogeneity of ADC including ion exchange chromatography (IEC), isoelectric focusing (IEF), and capillary electrophoresis (CE). This work aimed to give insights into the different analytical techniques used to determine pI and the charge distribution profile of ADCs.

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Additional details

Identifiers

DOI
10.54105/ijapsr.E4047.04050824
EISSN
2582-7618

Dates

Accepted
2024-08-15
Manuscript received on 05 July 2024 | Revised Manuscript received on 17 July 2024 | Manuscript Accepted on 15 August 2024 | Manuscript published on 30 August 2024.

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