Published August 27, 2024 | Version v1
Dataset Open

HUVEC CD44 siRNA perfusion tracking dataset

  • 1. ROR icon University of Turku
  • 2. ROR icon Åbo Akademi University

Description

This dataset contains tracking results of AsPC1 and MiaPaca cells perfused on CD44 siRNA-silenced endothelial monolayers under physiological flow speeds.

Videos were recorded using a Nikon Eclipse Ti2-E microscope and 20x objective.

Perfused cells from the generated videos were segmented using custom-trained Stardist models. Tracking was performed using TrackMate, and tracking results were analyzed using a custom CellTracksColab notebook. 

The dataset here contains the CSV files generated by TrackMate (Track and Spots information), the tracking data stored in the CellTracksColab format (Analysis.zip), and the analysis output used in the paper (Analysis.zip). 

Specifications

Contents of the repository

  • Analysis.zip

  • As_HUsi1.zip dataset

  • As_HUsi2.zip dataset

  • As_HUsi3.zip dataset

  • As_HUsiCtrl.zip dataset

  • Mia_HUsi1.zip dataset

  • Mia_HUsi2.zip dataset

  • Mia_HUsi3.zip dataset

  • Mia_HUsiCtrl.zip dataset

Reference

Fast label-free live imaging reveals key roles of flow dynamics and CD44-HA interaction in cancer cell arrest on endothelial monolayers
Gautier Follain, Sujan Ghimire, Joanna W. Pylvänäinen, Monika Vaitkevičiūtė, Diana Wurzinger, Camilo Guzmán, James RW Conway, Michal Dibus, Sanna Oikari, Kirsi Rilla, Marko Salmi, Johanna Ivaska, Guillaume Jacquemet
bioRxiv 2024.09.30.615654; doi: https://doi.org/10.1101/2024.09.30.615654

Files

Analysis.zip

Files (8.9 GB)

Name Size Download all
md5:1b1a0018fbbf617d28da3ba741310f5b
3.0 GB Preview Download
md5:ade451950cb4be555e82ad202da34f71
644.8 MB Preview Download
md5:d425a37580f586d62e37efdfd8fc6a0d
642.8 MB Preview Download
md5:5a68f4ca05ae9d7587a3a837d3a25ba0
526.5 MB Preview Download
md5:54a32ef09e46022bfa63ff5a94e74c1c
447.9 MB Preview Download
md5:71a8315ba4652f4329e00374d7db9ffa
1.2 GB Preview Download
md5:c8d77745dd878d8f906002e24daf221d
1.0 GB Preview Download
md5:8c6fee98f0dc5b8ce87f3a0c85c92002
1.4 GB Preview Download