Microbiota Study Crohn's Disease AG Chang
Authors/Creators
-
Budzinski, Lisa
(Work package leader)1, 2
- Chang, Hyun-Dong (Supervisor)1
- Sempert, Toni (Data collector)1
- Lietz, Leonie (Data collector)1
- Maier, René (Data collector)1
- Moser, Benjamin (Supervisor)3
- Büttner, Janine (Data collector)4
- Jessen, Bosse (Project leader)4
- Tóth, Marcell (Data collector)4
- Mashreghi, Mir-Farzin (Supervisor)1
-
1.
German Rheumatism Research Centre
- 2. Technical University Berlin
-
3.
DRK Kliniken Berlin
-
4.
Charité - Universitätsmedizin Berlin
Description
We have investigated a Crohn’s disease (CD) cohort by our multi-parameter microbiota flow cytometry approach to characterise the microbiota on single-cell level for attributes of the disease. The microbiota is isolated from stool samples and stained according to the published protocol for (a) host immunoglobulins IgA1, IgA2, IgM, IgG and (b) agglutinin binding to mannose, galactose or N-Acetyl-glucosamine surface sugar moieties. For all samples we also determined the microbiome composition by 16S rRNA (V3-V4) sequencing on the illumina MiSeq platform. We provide the raw .fcs and FASTQ files of 64 CD patients distributed into two cohorts (55) individuals in cohort 1, 19 individuals in cohort 2). Cohort 2 has two samples for two timepoints.
For comparison we additionally analysed 55 healthy donors.
All .fcs files were generated on BD Influx®.
The metadata is collected in the provided meta.csv.
The staining parameters are summarized in provided panel.csv.
Files
meta.csv
Files
(22.3 GB)
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Additional details
Related works
- Is variant form of
- Publication: 10.1002/eji.202250337 (DOI)
- References
- Book chapter: 10.1016/bs.mcb.2024.02.023 (DOI)
Funding
- Deutsche Forschungsgemeinschaft
- TRR241 B03 375876048 TRR241 B03
- Deutsche Forschungsgemeinschaft
- TRR241 B01 375876048
- European Union
- BacFlow - Analyse bakterieller Gemeinschaften durch Mikrobiota-Zytometrie EFRE-Project grant 1.6./01
- Deutsche Forschungsgemeinschaft
- 491069896 CRC1340
- Deutsche Forschungsgemeinschaft
- B04, Z02 CRC1449
- Deutsche Forschungsgemeinschaft
- 372486779 B06 CRC1340
- European Union
- 1.8/11 EFRE
- Dr. Rolf M. Schwiete Stiftung